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R&D Systems human duoset elisa kits
Human Duoset Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 372 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+ifn+gamma+duoset+elisa+kit/Human+IFN-gamma+DuoSet+ELISA/us12600779-1220-15-25
Average 96 stars, based on 372 article reviews

human duoset elisa kits - by Bioz Stars, 2026-09

96/100 stars

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Modification:

Article Title: Antibodies to ICOS
Article Snippet: .. The IFN-γ content in each well was determined using a modification of the Human IFN-gamma DuoSet ELISA kit (R&D systems). ..

Article Title: Antibodies to ICOS
Article Snippet: .. Measuring IFN-γ Levels The IFN-γ content in each well was determined using a modification of the Human IFN-gamma DuoSet ELISA kit (R&D systems). .. Capture antibody (50 μl/well) was coated overnight at 4 μg/ml in DPBS on black flat bottom, high binding plates (Greiner).

Enzyme-linked Immunosorbent Assay:

Article Title: Antibodies to ICOS
Article Snippet: .. The IFN-γ content in each well was determined using a modification of the Human IFN-gamma DuoSet ELISA kit (R&D systems). ..

Article Title: Immunomodulatory proteins with tunable affinities
Article Snippet: 10,000 matured DC and 100,000 purified allogeneic CD4+ T cells (BenTech Bio, USA) were co-cultured with ICOSL or CD80 variant Fc fusion proteins and controls in 96 well round bottom plates in 200 μl final volume of Ex-Vivo 15 media. .. On day 5, IFN-gamma secretion in culture supernatants was analyzed using the Human IFN-gamma Duoset ELISA kit (R&D Systems, USA). .. Optical density was measured by VMax ELISA Microplate Reader (Molecular Devices, USA) and quantitated against titrated rIFN-gamma standard included in the IFN-gamma Duo-set kit (R&D Systems, USA).

Article Title: Microengineered transplantation of human solid tumors for in vitro studies of CAR T immunotherapy
Article Snippet: .. Human IL-2 DuoSet ELISA kit (DY202-05, R&D Systems) and human IFN-gamma DuoSet ELISA kit (DY285B-05, R&D Systems) were used to measure the concentrations of IL-2 and IFNγ, respectively. ..

Article Title: Assessing the Value of T cell Monitoring in the General Population of Children and Adolescents – Insights From the Ciao Corona Cohort Study
Article Snippet: .. After incubation, stimulated supernatants were stored at −20 °C prior to analysis of IFN-gamma levels by ELISA assay (Human IFN-gamma DuoSet ELISA kit, R&D Systems, Catalog DY285B, and DuoSet ELISA Ancillary Reagent Kit 2, R&D Systems, Catalog DY008), following the manufacturer’s instructions. .. Samples were measured using a Multiskan SkyHigh Microplate Spectrophotometer with SkanIt software (Thermo Fisher Scientific) set to a wavelength of 450 nm with a 540 nm correction and IFN-gamma concentrations calculated from absorbance values by applying a 4 PL standard curve.

Article Title: A comparative analysis of CD70-directed CAR-T cells for glioblastoma treatment demonstrates a superior efficacy of the ligand-based construct
Article Snippet: .. The following kits were used to quantify cytokine secretion: Human Granzyme-B DuoSet enzyme-linked immunosorbent assay kit (ELISA; #DY2906–05, R&D Systems), Human IFN-gamma DuoSet ELISA kit (#DY285B, R&D Systems), Human TNF-alpha DuoSet ELISA kit (#DY210, R&D Systems), and Mouse TNF-alpha DuoSet ELISA kit (#DY410, R&D Systems). ..

other:

Article Title: CTLA-4 variant immunomodulatory proteins and uses thereof
Article Snippet: Optical density was measured on a BioTek Cytation Multimode Microplate Reader (BioTek Corp., USA) and quantitated against titrated rIFN-gamma standard included in the IFN-gamma Duo-set kit (R&D Systems, USA).

Article Title: Multi-domain immunomodulatory proteins and methods of use thereof
Article Snippet: Optical density is measured by VMax ELISA Microplate Reader (Molecular Devices, USA) and quantitated against titrated rIFN-gamma standard included in the IFN-gamma Duo-set kit (R&D Systems, USA).

Incubation:

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In vitro assessment of the CD70-directed CAR-T cell anti-GB cytotoxicity (A) Measurement of secreted TNF-α and IFN-γ in the SN of GB/CAR-T cell co-cultures by ELISA. N = 3 biological replicates per group. For comparison between MCS and CD70 (upper bar plots), an unpaired two-tailed t test was used. For comparisons among constructs (bottom), a one-way ANOVA followed by a Holm-Šídák multiple comparisons test was used. (B) Measurement of tumor cell signal during co-culture with CAR-T cells on the Incucyte platform. N = 2 biological replicates per group. Every biological replicate is the mean of N = 5 technical replicates. Time point intervals = 45 min. A two-tailed Student’s t test was performed using the values of the last measured time point to determine statistical significance. For (A) and (B), data presented as mean (SD). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; n.s., not significant.

Journal: Molecular Therapy Oncology

Article Title: A comparative analysis of CD70-directed CAR-T cells for glioblastoma treatment demonstrates a superior efficacy of the ligand-based construct

doi: 10.1016/j.omton.2026.201134

Figure Lengend Snippet: In vitro assessment of the CD70-directed CAR-T cell anti-GB cytotoxicity (A) Measurement of secreted TNF-α and IFN-γ in the SN of GB/CAR-T cell co-cultures by ELISA. N = 3 biological replicates per group. For comparison between MCS and CD70 (upper bar plots), an unpaired two-tailed t test was used. For comparisons among constructs (bottom), a one-way ANOVA followed by a Holm-Šídák multiple comparisons test was used. (B) Measurement of tumor cell signal during co-culture with CAR-T cells on the Incucyte platform. N = 2 biological replicates per group. Every biological replicate is the mean of N = 5 technical replicates. Time point intervals = 45 min. A two-tailed Student’s t test was performed using the values of the last measured time point to determine statistical significance. For (A) and (B), data presented as mean (SD). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; n.s., not significant.

Article Snippet: The following kits were used to quantify cytokine secretion: Human Granzyme-B DuoSet enzyme-linked immunosorbent assay kit (ELISA; #DY2906–05, R&D Systems), Human IFN-gamma DuoSet ELISA kit (#DY285B, R&D Systems), Human TNF-alpha DuoSet ELISA kit (#DY210, R&D Systems), and Mouse TNF-alpha DuoSet ELISA kit (#DY410, R&D Systems).

Techniques: In Vitro, Enzyme-linked Immunosorbent Assay, Comparison, Two Tailed Test, Construct, Co-Culture Assay

Evaluation of CD70-directed CAR-T cell effector function in cerebral organoids (A) Confocal microscopy of cerebral organoids, infiltrated by generated GB models. (B) Quantification of CD70 signal in organoids from (A). Each dot represents an organoid. A Welch’s t test was used to assess significance. (C) Immunofluorescence analysis of endogenous CD70 expression in cerebral organoids. (D) Confocal microscopy of cerebral organoids previously invaded by GB cells and subsequently treated with CAR-T cells for 3 d. (E) Quantification of Granzyme-B signal from (D). A two-tailed t test was used to determine significance. (F) Measurement of secreted Granzyme-B and IFN-γ levels in the SN of co-cultures from (D) by ELISA. N = 3 biological replicates per group. (G) CAR construct direct comparisons from (F). A one-way ANOVA followed by a Tukey’s post hoc test for multiple comparisons was used. For (A), (C), and (D), scale bars, 200 μm. For (D) and (E), N ≥ 3 organoids per group. For (E) and (F), a two-tailed t test was used to assess significance. For (B), (E), and (F), data presented as mean (SD). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; n.s., not significant.

Journal: Molecular Therapy Oncology

Article Title: A comparative analysis of CD70-directed CAR-T cells for glioblastoma treatment demonstrates a superior efficacy of the ligand-based construct

doi: 10.1016/j.omton.2026.201134

Figure Lengend Snippet: Evaluation of CD70-directed CAR-T cell effector function in cerebral organoids (A) Confocal microscopy of cerebral organoids, infiltrated by generated GB models. (B) Quantification of CD70 signal in organoids from (A). Each dot represents an organoid. A Welch’s t test was used to assess significance. (C) Immunofluorescence analysis of endogenous CD70 expression in cerebral organoids. (D) Confocal microscopy of cerebral organoids previously invaded by GB cells and subsequently treated with CAR-T cells for 3 d. (E) Quantification of Granzyme-B signal from (D). A two-tailed t test was used to determine significance. (F) Measurement of secreted Granzyme-B and IFN-γ levels in the SN of co-cultures from (D) by ELISA. N = 3 biological replicates per group. (G) CAR construct direct comparisons from (F). A one-way ANOVA followed by a Tukey’s post hoc test for multiple comparisons was used. For (A), (C), and (D), scale bars, 200 μm. For (D) and (E), N ≥ 3 organoids per group. For (E) and (F), a two-tailed t test was used to assess significance. For (B), (E), and (F), data presented as mean (SD). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; n.s., not significant.

Techniques: Confocal Microscopy, Generated, Immunofluorescence, Expressing, Two Tailed Test, Enzyme-linked Immunosorbent Assay, Construct

mCD27-based anti-murine CD70 CAR-T cells are potent against murine GB in vitro (A) Murine CD27-based construct design. (B) Transduction efficiency of primary murine T cells by flow cytometry. A non-transduced (NT) sample from each donor mouse was used to determine gating. Data gated on single live mCD3 + cells. (C) Measurement of mCD70 gene expression levels in generated OE models by RT-qPCR. N = 3 technical replicates per cell line. (D) Measurement of mCD70 on the surface of generated murine OE GB models by flow cytometry (blue histograms). Signal was compared to that of an isotype control (red histograms). (E) Quantification of secreted TNF-α in the SN of mGB/mCAR-T cell co-cultures by ELISA. N = 3 biological replicates per group. (F) Pairwise comparisons of secreted TNF-α levels from (E) among targeting constructs. A one-way ANOVA with a post hoc Holm-Šídák test was used for significance. (G) Schematic representation of the live-cell imaging pipeline. (H) Quantification of tumor cell signal from (G) over time. A one-way ANOVA with a Dunnett’s multiple comparisons test was used with data from the t = 660 min mark. For (C) and (E), an unpaired two-tailed t test was used for significance. Data are presented as mean (SD). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; n.s., not significant; n.d., not detected.

Journal: Molecular Therapy Oncology

Article Title: A comparative analysis of CD70-directed CAR-T cells for glioblastoma treatment demonstrates a superior efficacy of the ligand-based construct

doi: 10.1016/j.omton.2026.201134

Figure Lengend Snippet: mCD27-based anti-murine CD70 CAR-T cells are potent against murine GB in vitro (A) Murine CD27-based construct design. (B) Transduction efficiency of primary murine T cells by flow cytometry. A non-transduced (NT) sample from each donor mouse was used to determine gating. Data gated on single live mCD3 + cells. (C) Measurement of mCD70 gene expression levels in generated OE models by RT-qPCR. N = 3 technical replicates per cell line. (D) Measurement of mCD70 on the surface of generated murine OE GB models by flow cytometry (blue histograms). Signal was compared to that of an isotype control (red histograms). (E) Quantification of secreted TNF-α in the SN of mGB/mCAR-T cell co-cultures by ELISA. N = 3 biological replicates per group. (F) Pairwise comparisons of secreted TNF-α levels from (E) among targeting constructs. A one-way ANOVA with a post hoc Holm-Šídák test was used for significance. (G) Schematic representation of the live-cell imaging pipeline. (H) Quantification of tumor cell signal from (G) over time. A one-way ANOVA with a Dunnett’s multiple comparisons test was used with data from the t = 660 min mark. For (C) and (E), an unpaired two-tailed t test was used for significance. Data are presented as mean (SD). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; n.s., not significant; n.d., not detected.

Techniques: In Vitro, Construct, Transduction, Flow Cytometry, Gene Expression, Generated, Quantitative RT-PCR, Control, Enzyme-linked Immunosorbent Assay, Live Cell Imaging, Two Tailed Test

PSMA CAR T cells effectively eradicated PSMA-positive rhabdoid cells in vitro (A) Schematic diagram of the PSMA CAR T vector. (B) Expression of PSMA CAR in primary T cells as determined by western blot analysis using a CD3ζ antibody. The arrow marks the endogenous CD3ζ. (C) Flow cytometry analysis assessing cell surface expression of PSMA CAR on engineered CAR T cells. (D) In vitro cytotoxicity assay. Luciferase-expressing rhabdoid cells were incubated with PSMA CAR T cells or non-transduced T cells at various target-to-effector (T:E) ratios. p values were calculated by comparing each condition with TTC1240 at the corresponding T:E ratio. Group differences were evaluated using multiple unpaired t tests with FDR correction. N = 3. (E) INF-γ levels were measured by ELISA using the same experimental setup as in (D).

Journal: Molecular Therapy Oncology

Article Title: Rhabdoid tumors as a novel target for PSMA-directed CAR T cell therapy

doi: 10.1016/j.omton.2026.201125

Figure Lengend Snippet: PSMA CAR T cells effectively eradicated PSMA-positive rhabdoid cells in vitro (A) Schematic diagram of the PSMA CAR T vector. (B) Expression of PSMA CAR in primary T cells as determined by western blot analysis using a CD3ζ antibody. The arrow marks the endogenous CD3ζ. (C) Flow cytometry analysis assessing cell surface expression of PSMA CAR on engineered CAR T cells. (D) In vitro cytotoxicity assay. Luciferase-expressing rhabdoid cells were incubated with PSMA CAR T cells or non-transduced T cells at various target-to-effector (T:E) ratios. p values were calculated by comparing each condition with TTC1240 at the corresponding T:E ratio. Group differences were evaluated using multiple unpaired t tests with FDR correction. N = 3. (E) INF-γ levels were measured by ELISA using the same experimental setup as in (D).

Article Snippet: Supernatants collected from the in vitro cytotoxicity assay were used to quantify IFN-γ secretion by T cells or CAR T cells using the Human IFN-γ DuoSet ELISA kit (R&D Systems, DY285B).

Techniques: In Vitro, Plasmid Preparation, Expressing, Western Blot, Flow Cytometry, Cytotoxicity Assay, Luciferase, Incubation, Enzyme-linked Immunosorbent Assay

PRED suppresses human CD8 + effector T-cell activation and limits autologous melanoma killing in vitro . (a) Melanoma cells (SMU17-0263) were co-cultured with autologous CD8⁺ tumor-infiltrating lymphocytes (TILs) for 72 hours in the presence of PRED at indicated concentrations. Images were captured using the IncuCyte system. Scale bar, 100 μm. (b-c) Flow cytometry analysis of melanoma and T cell content (b) and T-cell activation (c) in the presence of PRED. Relative content of viable melanoma and CD8 + T-cells and melanoma/TIL ratio (b) normalized to control (DMSO). (c) Downregulation of T-cell activation markers CD69 and/or CD137 (4-1BB) following PRED exposure (c). Data represent mean ± SD of five independent co-culture experiments performed in duplicate or triplicate. Statistical analysis was with one-way ANOVA with Dunnett's multiple comparisons; significant p -values are shown. (d) IFNγ levels in supernatants collected 72 hours after co-culture of SMU17-0263 melanoma cells with autologous TILs were measured by ELISA. ELISA absorbance (OD) values were blank-subtracted and converted to pg/mL by interpolation from standard curve (4-parameter logistic fit). Bars show mean ± SD, dots indicate biological replicates ( n =5 per group). Statistical analysis was conducted using ordinary one-way ANOVA with Dunnett’s multiple comparisons vs DMSO; significant p -values are shown.

Journal: Oncoimmunology

Article Title: Prednisolone modulates CD8⁺ and regulatory T-cell activity to dampen response to immune checkpoint inhibitor therapy in melanoma

doi: 10.1080/2162402X.2026.2643494

Figure Lengend Snippet: PRED suppresses human CD8 + effector T-cell activation and limits autologous melanoma killing in vitro . (a) Melanoma cells (SMU17-0263) were co-cultured with autologous CD8⁺ tumor-infiltrating lymphocytes (TILs) for 72 hours in the presence of PRED at indicated concentrations. Images were captured using the IncuCyte system. Scale bar, 100 μm. (b-c) Flow cytometry analysis of melanoma and T cell content (b) and T-cell activation (c) in the presence of PRED. Relative content of viable melanoma and CD8 + T-cells and melanoma/TIL ratio (b) normalized to control (DMSO). (c) Downregulation of T-cell activation markers CD69 and/or CD137 (4-1BB) following PRED exposure (c). Data represent mean ± SD of five independent co-culture experiments performed in duplicate or triplicate. Statistical analysis was with one-way ANOVA with Dunnett's multiple comparisons; significant p -values are shown. (d) IFNγ levels in supernatants collected 72 hours after co-culture of SMU17-0263 melanoma cells with autologous TILs were measured by ELISA. ELISA absorbance (OD) values were blank-subtracted and converted to pg/mL by interpolation from standard curve (4-parameter logistic fit). Bars show mean ± SD, dots indicate biological replicates ( n =5 per group). Statistical analysis was conducted using ordinary one-way ANOVA with Dunnett’s multiple comparisons vs DMSO; significant p -values are shown.

Article Snippet: IFNγ levels in co-culture supernatants were quantified using a human IFNγ DuoSet ELISA kit (R&D Systems, Cat# DY285B) as per manufacturer’s instructions.

Techniques: Activation Assay, In Vitro, Cell Culture, Flow Cytometry, Control, Co-Culture Assay, Enzyme-linked Immunosorbent Assay

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